A student answer sheet and teacher Power Point are attached for use during this lab. This activity was adapted from An Introduction of Biotechnology developed by the Mathematics and Science Education Center, University of Missouri- St. The use of methylene blue gives them a visual model of how samples become less concentrated with each dilution step. The exercise will allow students to learn how to pipette, dilute samples and calculate the concentration of their samples. Pre Lab: Serial Dilutions Practice Exercise If students are not familiar with operating pipettes, the Pre Lab Serial Dilutions practice exercise is recommended. Dilutions Lab Question: Using a certain amount of solid CuSO4, how can we perform a serial dilution to attain known concentrations? Materials Needed: - Distilled water Step 1: In the 50 ml volumetric flask, dissolve your calculated amount of CuSO4, in enough water to make a 50ml solution. This is because it is hard to count more than 300 colonies on an agar plate if we inoculated directly from. Before doing plate counts, serial dilutions are required. Answer to I'm writing a Bio lab report about serial dilutions. Conclusion We can determine the number of microorganisms in a sample. Serial dilutions of a sample are spread onto the.

Serial dilution lab conclusion